Rice Genetics Newsletter, Vol. 6
Edited by H. I. Oka and G. S. Khush
A Publication of
Rice Genetics Cooperative
December 1989
Genetic Resources Section
National Institute of Genetics
Mishima, Japan
Table of Contents
Cover, p. 0
Office Bearers, p. i
A. Report of the Coordinating Committee of Rice Genetics Cooperative
(by M. S. Swaminathan and T. Matsuo), p. 1
B. Report of the Committee on Gene Symbolization, Nomenclature and
Linkage Groups (by T. Kinoshita)
I. Proposal for rice gene symbols, p. 2
II. Registration of new gene symbols, p. 2
III. List of new gene symbols and literature on genic analysis, p. 7
IV. List of gene symbols recommended (with linkage group and
key literature), p. 12
C. Report of the Committee on Genetic Stocks (by T. Omura), p. 30
List of marker genes (Supplement, by T. Kinoshita)
D. Report of the Committee on Genetic Engineering (by Ray Wu), p. 32
E. List of Publications on Rice Genetics, (Supplement, compiled
by F. Kikuchi), p. 38
F. Research Notes (List of contents on next page), p. 50
G. Mailing List (Supplement, compiled by Y. Futsuhara), p. 178
Editors' Note (by H. I. Oka and G. S. Khush), p. 180
F. Research Notes
I. Cytological studies
1. Establishment of a trisomic series in rice by using a desynaptic
mutant, by H. Yasui, H. Satoh and N. Iwata, p. 50
2. Induction of X + 1 plants of rice by means of anther culture of
trisomic plants, by Zi-Xuan Wang, Y. Oda and N. Iwata, p. 52
3. Karyotype analysis of Lu-Dao , an indigenous weedy rice germplasm
found in Jiangsu, China, by Zeng-Jian Chen and Li-Hong Zhu54
4. Comparison of length between chromosomes 2 and 3, by H. I. Oka.56
II. Interspecific crosses
5. Crossability of wild Oryza species and their potential use for
improvement of cultivated rice, by L. A. Sitch, R. D. Dalmacio
and G. 0. Romero, p. 58
6. An Oryza sativa X 0. officinalis hybrid obtained by using a
male-sterile line as maternal parent, by Weii-Jin Yu, p. 60
III. Evolution and varietal differentiation
7. A supplementary note on the sickle-shaped rice cultivars, by K. S.
Cheng, N. W. Huang and Y. Z. Zhang, p. 61
8. Genetic divergence among rices from Northeast India, by J. C.
Glaszmann, P. Benyayer and M. Arnaud, p. 63
9. Two SDS-PAGE protein bands specific to subspecies indica and japo-
nica of rice, by S. M. Zhang, C. Bai and L. S. Liu, p. 65
10. Plant opal analysis as a method of distinguishing between subspecies
indica and japonica of 0. sativa, by Y. I. Sato, H. Fujiwara, T.
Udatsu and H. Morishima, p. 67
11. Distribution of gene diversity in Indica and Japonica rice varieties
and their wild progenitors, by H. I. Oka, p. 70
12. Variation and inheritance of seed shedding in weedy rice, by Lin-Hua
Tang and H. Morishima, p. 72
13. Additional observations and collection of the Oryza latifolia Desv.
species complex, by G. Second, p. 73
14. Phylogenetic analysis of the tribe Oryzeae: Total chloroplast DNA
restriction fragment analysis, by S. H. Zhang and G. Second, p. 76
IV. Segregation distortion
15. Abnornial segregation patterns in crosses between Oryza savita and
0. glaberrima, by J. L. Pham and B. Bougerol, p. 80
16. Distorted F2 ratios and changes in gene frequency observed for the
wx and other three loci, by H. I. Oka, p. 83
17. Estimation of recombination values between loci C and wx from
distorted F2 segregation data, by H. I. Oka, p. 86
18. Comparative gametic selection in sexual and anther culture
derivatives from five distant F, hybrids of rice, by E.
Guiderdoni, p. 91
V. New genes and linkages
19. Gamete eliminator detected in the wild progenitor of Oryza sativa,
by Y.Sano, p. 93
20. An intermediate amylose content determined by an allele at the wx
locus, by M. Nishiniura and Y. Sano, p. 95
21. Gene Lk-f for long grain found in an induced mutant of variety
Shiokari, by T. Kinoshita, H. Kikuchi and 1. Takamure, p. 96
22. An induced dwarfing gene , sd-7(t), obtained in Taichung 65, by K.
H. Tsai, p. 99
23. Allelism of genes for elongation of uppermost internode from two
different sources, by M. Maekawa, T. Maekawa, N. Shinbashi and T.
Kinoshita, p. 101
24. Identification of four new isozyme loci in rice, by B. G. delos
Reyes, D. S. Brar and G. S. Khush, p. 103
25. Linkage map for Nishimura's chromosome 8, by N. Iwata, H. Satoh
and A. Yoshimura, p. 106
26. Location of a mutant gene for rice storage protein esp-3 on Nishi-
mura's chromosome 9, by T. Kumamaru, H. Satoh, T. Omura and M.
Ogawa, p. 108
27. Linkage analysis for four isozyme loci , Adh-1, Acp-1, Pox-2 and Pgd-
2, by R. Ishikawa and H. Morishima, p. 109
VI. Physiological genetics
28. Genetics of leaf rolling under vegetative stage drought stress, by
B. N. Singh and D. J. Mackill, p. 112
29. Genetic control of iron deficiency chlorosis tolerance in rice, by C.
D. R. Reddy, Y. S. Nerkar and M. B. Misal, p. 115
30. A temperature-sensitive male-sterile line found in rice, by X. Z.
Sun, S. K. Min and Z. M. Xiong, p. 116
31. Differential pollen tube elongation causing "alien pollen primacy"
in rice, by Y. I. Sato, p. 117
32. Developmental changes in transcription activity of genes in rice
seed, by X. H. Zhang and X. H. Tang, p. 119
VII. Genes for cold tolerance and disease resistance
33. Number of effective factors controlling cold tolerance at the
anthesis stage, by M. Nishimura and K. Hamamura, p. 122
34. Location of a new gene for resistance to bacterial blight found in
Yunnan local variety Haomei, by L. Y. Dai, D. P. Zhang and Y.
F. Xie, p. 124
VIII. Cytoplasmic male sterility and related studies
35. Identifying potential sources of cytoplasmic male sterility in rice,
by L. A. Sitch and R. D. Dalmacio, p. 125
36. Cytoplasmic diversity among CMS lines of rice, by S. S. Virmani, R.
Vijaya Kumar and C. Casal, p. 127
37. Identification of wide compatibility genes in rice, by S. S.
Virmani, R. Vijaya Kumar and P. S. Aurin, p. 129
IX. Mutation and gene action
38. Temperature sensitive chlorophyll mutations , by 0. Yatou and X. Y.
Cheng, p. 131
39. Formation of abscission layer controlled by a shattering gene linked
with semidwarfing gene sd-1 in rice,by S. Oba and F. Kikuchi.132
40. Effects of dwarfing genes on proteins in rice grain observed in
isogenic lines of variety Shiokari, by K. Watanabe and 0.
Kamijima, p. 134
41. Effects of dwarfing genes on coleoptile and mosocotyl elongation ob-
served in isogenic lines of variety Shiokari, by K. Watanabe and 0.
Kamijima, p. 135
42. Protein kinase activity in diverse tissues of two isogenic lines of
rice, by C. M. Cheng, K. H. Tsai and N. T. Hu, p. 137
X. Tissue and cell culture
43. Mutagenesis by means of anther culture combined with gamma ir-
radiation, by T. Kinoshita, K. Mori and 1. Takamure, p. 139
44. In vitro screening of the effects of antimitotics on differentiating
rice tissues, by A. N. Sharma and U. Sinha, p. 141
45. A female-sterile mutant found in a somaclonal line for IR50, by D.
H. Ling, Z. R. Ma, M. F. Chen and W. Y. Chen, p. 143
XI. Molecular genetics
46. The chloroplast-encoded ORF39 sequence of Oryza rufipogon, by Z.
X. Luo, X. P. Zhao, J. C. Cote and Ray Wu, p. 144
47. Comparison of eleven chloroplast genes between an Indica and
a Japonica cultivar of rice, by E. C. Kemmerer and Ray Wu147
48. Occasional biparental inheritance of chloroplast DNA in rice, by G.
Second, A. Dally and S. H. Zhang, p. 150
49. RFLP analysis of rice mitochondrial DNA , by T. Ishii and K. Tsune-
waki, p. 153
50. Heterogeneity of small plasmid-like DNAs found in rice mitochon-
dria, by W. Sakamoto, K. Kadowaki, K. Harada, S. Tano and H.
Yamaguchi, p. 155
51. Cloning and sequencing of mitochondrial encoded genes irt rice, by
E. K. Kalelkau, C. Andre;, B. Doshi and V. Walbot, p. 157
52. DNA structure of mutant genes: An observation of the wx locus, by
0. Yatou, K. Kadowaki and E. Amano, p. 157
53. Transgenic rice plants produced from protoplasts following direct
uptake of Bacillus thuringiensis gamma-endotoxin protein gene, by H.
Yang, S. D. Guo, J. X. L], X. J. Chen and Y. L. Fan, p. 159
54. Cloning and comparison of the atpA gene in mtDNA between male-
sterile and normal rice lines, by D. D. Li and B. Wang, p. 161
XII. Technical notes
55. Isolation of RNA and DNA from different rice tissues, by L. A. N.
Hensgens and P. E. Van Os-Ruygrok, p. 163
56. Improved histochemical staining for Beta-D-glucuronidase activity in
monocotyledonous plants, by S. Rueb and L. A. M. Hensgens168
57. Screening of totipotent rice cell line for efficient transformation,
by A. P. Aryan an,d T. W. Okita, p. 170
58. High frequency of protoplast division and regeneration in Indica
rice, by B. L. Sun, X.H. Li, Y.R. Sun, S.M. Zhao and J.Chen..170
59. Plant regeneration from protoplasts of Indica and Japonica rices, by
X. H. Li, A. Aryan, C. K. Xiang and T. Okita, p. 173
60. High regeneration frequency of protoplasts obtained in rice variety
Zhonghua 8, by J. X. Li, H. Yang and Y. L. Fan, p. 175
61. A rapid, reliable method for preparing somatic chromosomes of rice,
by N. Islam-Faridi and L. A. Sitch, p. 176