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E.g., Wessler, regeneration, PubMed ID 17578919.

expand all sections collapse all sections  Reference "A physical, genetic and functional sequence assembly of the barley genome"
Reference ID 54955
Title A physical, genetic and functional sequence assembly of the barley genome
Source Nature, 2012, vol. 491, pp. 711-716
Authors (72)
Abstract Barley (Hordeum vulgare L.) is among the world's earliest domesticated and most
important crop plants. It is diploid with a large haploid genome of 5.1
gigabases (Gb). Here we present an integrated and ordered physical, genetic and
functional sequence resource that describes the barley gene-space in a
structured whole-genome context. We developed a physical map of 4.98 Gb, with
more than 3.90 Gb anchored to a high-resolution genetic map. Projecting a deep
whole-genome shotgun assembly, complementary DNA and deep RNA sequence data onto
this framework supports 79,379 transcript clusters, including 26,159 'high-
confidence' genes with homology support from other plant genomes. Abundant
alternative splicing, premature termination codons and novel transcriptionally
active regions suggest that post-transcriptional processing forms an important
regulatory layer. Survey sequences from diverse accessions reveal a landscape of
extensive single-nucleotide variation. Our data provide a platform for both genome-
assisted research and enabling contemporary crop improvement.
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